Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: The relationship between PD-1 expression and malignancy

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: High PD-1 expression in tissues of patients with HPC predicts a worse prognosis. A PD-1 is indicated by green fluorescence, and CD8 is indicated by red fluorescence. Scale: 80 µm. Shown at 100× original magnification. B Expression of PD-1 in tumor and paracancerous tissues ( P < 0.001). C , D Kaplan–Meier survival curves were used to analyze the relationship between PD-1 expression and OS and DFS ( P < 0.001, P = 0.0011)

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques: Expressing, Fluorescence

Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: Univariate analysis to assess the association of clinicopathological parameters with prognosis of HPC

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques:

Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: Multivariate analysis to assess the association of clinicopathological parameters with prognosis of HPC

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques: Cell Differentiation

Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: Serum-derived exosomes can be taken up by CD8+ T cells. A Representative TEM images of purified exosomes from sera of patients with HPC. Scale: 200 nm. B Immunoblotting of CD63 expression in whole cell lysates and purified exosomes. C The exosomes were identified by TEM. D The exosomes could be taken up by CD8+ T cells. The exosomes were stained with PKH-69 (green), and the nuclei were stained with DAPI (blue). The red arrow indicates the exosomes. Scale: 50 nm

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques: Derivative Assay, Purification, Western Blot, Expressing, Staining

Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: Serum-derived exosomes can affect the function of CD8+ T cells. A – C Changes in PD-1 expression in CD8+ T cells stimulated with PBS, control serum exosomes and patient serum exosomes were observed. D The serum-derived exosomes of patients significantly increased the expression of PD-1 in CD8+ T cells ( P < 0.001). E , F The cell killing ability of T cells was determined by CCK-8 assay ( P = 0.049). G , H The levels of IL-10 and TGF-β in the medium were determined by ELISA ( P < 0.001, P < 0.001; P < 0.001, P = 0.393)

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques: Derivative Assay, Expressing, Control, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

Journal: Cancer Cell International

Article Title: Serum-derived exosomes promote CD8+ T cells to overexpress PD-1, affecting the prognosis of hypopharyngeal carcinoma

doi: 10.1186/s12935-021-02294-z

Figure Lengend Snippet: The serum-derived exosomes of patients protect HPC cells from CD8+ T cells through up-regulation of PD-1

Article Snippet: Then, 2 μl of PD-1 and 2 μl of CD8 flow cytometry antibodies were added and incubated on ice at 4 °C for 30 min. Flow cytometry (Becton, Dickinson and Company, America) was used to analyze the cellular immunophenotype, and the data were analyzed.

Techniques: Derivative Assay